Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads.
Identifieur interne : 000394 ( 2020/Analysis ); précédent : 000393; suivant : 000395Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads.
Auteurs : Yang Pan [République populaire de Chine] ; Luyao Long [République populaire de Chine] ; Daitao Zhang [République populaire de Chine] ; Tingting Yan [République populaire de Chine] ; Shujuan Cui [République populaire de Chine] ; Peng Yang [République populaire de Chine] ; Quanyi Wang [République populaire de Chine] ; Simei Ren [République populaire de Chine]Source :
- Clinical chemistry [ 1530-8561 ] ; 2020.
Abstract
Corona Virus Disease-2019 (COVID-19) has spread widely throughout the world since the end of 2019. Nucleic acid testing (NAT) has played an important role in patient diagnosis and management of COVID-19. In some circumstances, thermal inactivation at 56 °C has been recommended to inactivate Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) before NAT. However, this procedure could theoretically disrupt nucleic acid integrity of this single-stranded RNA virus and cause false negatives in real-time polymerase chain reaction (RT-PCR) tests.
DOI: 10.1093/clinchem/hvaa091
PubMed: 32246822
Affiliations:
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R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences</wicri:regionArea><wicri:noRegion>Chinese Academy of Medical Sciences</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Daitao" sort="Zhang, Daitao" uniqKey="Zhang D" first="Daitao" last="Zhang">Daitao Zhang</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yan, Tingting" sort="Yan, Tingting" uniqKey="Yan T" first="Tingting" last="Yan">Tingting Yan</name><affiliation wicri:level="3"><nlm:affiliation>Department of Clinical Laboratory, Beijing Hospital, National Center of Gerontology, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Beijing Hospital, National Center of Gerontology, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cui, Shujuan" sort="Cui, Shujuan" uniqKey="Cui S" first="Shujuan" last="Cui">Shujuan Cui</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yang, Peng" sort="Yang, Peng" uniqKey="Yang P" first="Peng" last="Yang">Peng Yang</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Quanyi" sort="Wang, Quanyi" uniqKey="Wang Q" first="Quanyi" last="Wang">Quanyi Wang</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ren, Simei" sort="Ren, Simei" uniqKey="Ren S" first="Simei" last="Ren">Simei Ren</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, P. R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences</wicri:regionArea><wicri:noRegion>Chinese Academy of Medical Sciences</wicri:noRegion></affiliation></author></analytic><series><title level="j">Clinical chemistry</title><idno type="eISSN">1530-8561</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Corona Virus Disease-2019 (COVID-19) has spread widely throughout the world since the end of 2019. Nucleic acid testing (NAT) has played an important role in patient diagnosis and management of COVID-19. In some circumstances, thermal inactivation at 56 °C has been recommended to inactivate Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) before NAT. However, this procedure could theoretically disrupt nucleic acid integrity of this single-stranded RNA virus and cause false negatives in real-time polymerase chain reaction (RT-PCR) tests.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Pan, Yang" sort="Pan, Yang" uniqKey="Pan Y" first="Yang" last="Pan">Yang Pan</name></noRegion><name sortKey="Cui, Shujuan" sort="Cui, Shujuan" uniqKey="Cui S" first="Shujuan" last="Cui">Shujuan Cui</name><name sortKey="Long, Luyao" sort="Long, Luyao" uniqKey="Long L" first="Luyao" last="Long">Luyao Long</name><name sortKey="Ren, Simei" sort="Ren, Simei" uniqKey="Ren S" first="Simei" last="Ren">Simei Ren</name><name sortKey="Wang, Quanyi" sort="Wang, Quanyi" uniqKey="Wang Q" first="Quanyi" last="Wang">Quanyi Wang</name><name sortKey="Yan, Tingting" sort="Yan, Tingting" uniqKey="Yan T" first="Tingting" last="Yan">Tingting Yan</name><name sortKey="Yang, Peng" sort="Yang, Peng" uniqKey="Yang P" first="Peng" last="Yang">Peng Yang</name><name sortKey="Zhang, Daitao" sort="Zhang, Daitao" uniqKey="Zhang D" first="Daitao" last="Zhang">Daitao Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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